Fast assembly of non-thiolated DNA on gold surface at lower pH
نویسندگان
چکیده
منابع مشابه
Fast assembly of non-thiolated DNA on gold surface at lower pH.
In a typical protocol for attaching DNA to a gold electrode, thiolated DNA is incubated with the electrode at neutral pH overnight. Here we report fast adsorption of non-thiolated DNA oligomers on gold electrodes at acidic pH (i.e., pH ~3.0). The peak-to-peak potential difference and the redox peak currents in typical cyclic voltammetry of [Fe(CN)6](3-) are investigated to monitor the attachmen...
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Thiols have a strong tendency to be chemisorbed to gold surfaces forming self-assembled monolayers. 2] The feature of strong S Au bond formation has been exploited in numerous applications including the immobilisation of DNA on gold surfaces for the generation of DNA microarrays or gold electrodes for measuring charge migration mediated by double stranded DNA. To be immobilised on gold, DNA has...
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DNA-based nano-biosensors are emerging scope in the field of biosensors. Many synthetic single stranded functional DNAs have been applied for development of such sensors, recently. Immobilization of DNA oligonucleotides on the surface of gold nanoparticles is a key step in the development of most colorimetric nano-biosensors. The bound DNA is usually thiolated and forms Au-S covalent bond to th...
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ژورنال
عنوان ژورنال: Journal of Colloid and Interface Science
سال: 2013
ISSN: 0021-9797
DOI: 10.1016/j.jcis.2013.08.043